英语翻译The propensity for 1 to bind to DNA and also selectivelyaccumulate within cancer cells in an intact manner promptedus to investigate its uptake and bio-distribution withinindividual A549 human lung carcinoma cells and also itseffect on en

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英语翻译The propensity for 1 to bind to DNA and also selectivelyaccumulate within cancer cells in an intact manner promptedus to investigate its uptake and bio-distribution withinindividual A549 human lung carcinoma cells and also itseffect on en
英语翻译
The propensity for 1 to bind to DNA and also selectively
accumulate within cancer cells in an intact manner prompted
us to investigate its uptake and bio-distribution within
individual A549 human lung carcinoma cells and also its
effect on endogenous elements by means of synchrotron XRF
(X-ray fluorescence) imaging methods.Figure 1 shows elemental
distribution maps for a single A549 cancer cell treated
with 5 mm of 1 for 24 h.The most significant result is that high
levels of both gadolinium and platinum are co-localized with
the intense region in the zinc and phosphorus maps,which is
assumed to define the nucleus.These results are consistent
with the cellular uptake of 1 followed by its accumulation in
the nucleus,and the same result was observed in all five cells
measured (Supporting Information,Figures S2–S6).The fact
that the gadolinium and platinum maps are essentially
identical indicates that 1 remains largely intact throughout
the uptake and accumulation processes,which is consistent
with the results of the ICP-MS experiments (see above).
Further support is given by the fact that the XRF-determined
molar ratio of platinum to gadolinium in the cell matches the
stoichiometry of the complex within experimental error (2:1;
Supporting Information,Table S3).

英语翻译The propensity for 1 to bind to DNA and also selectivelyaccumulate within cancer cells in an intact manner promptedus to investigate its uptake and bio-distribution withinindividual A549 human lung carcinoma cells and also itseffect on en
1与DNA结合的倾向和在癌细胞中以完整的方式选择性地积聚,促使我们用同步X射线荧光（XRF）成像法来研究其在各别A549人肺癌细胞中的摄入和生物分布,以及其对内源性元素的影响.图1示出了用5μM的1处理24h的单一A549癌细胞的元素分布地图.最有意义的结果是,高水平的Gd和Pt与锌和亚磷地图的密集区共同局部化,这被假设定义了原子核.这些结果与1在其于原子核中的聚集后的细胞摄入相一致,而且在全部5种被测量的细胞中都观察到了相同的结果（支持信息S2－S6）.Gd和Pt地图在本质上是相同的这一事实表明,1在整个摄入和积聚过程中多半保持完整,这与ICP-MS实验的结果（见上面）是一致的.XRF确定的细胞中Pt与Gd的摩尔比与络合物的化学计量比在实验误差范围内是匹配的（2：1；支持信息,表S3）.

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